CCAFS’ engagement with the Central America Agricultural Council and the Council of Ministers of Women and a demand-driven process involving the Gender and Climate Change Units at the Ministry of Agriculture, Livestock and Food and other 22 organizations led to the participatory development of a Step-by-step guide on how to mainstream gender along the different phases of Climate-Smart-Agriculture programs and policies. This practical translation of CCAFS gender research will foster new actions on the ground in Guatemala and the region.