Effects of Freeze Drying on Antioxidants and Immunoglobulins Level of Zebu Bovine Colostrum

Abstract

The aim of present study was to investigate the effect of freeze drying on total antioxidant and immunoglobulin levels of bovine colostrum in desi breed (Zebu cattle). A total of 12 colostrum samples were collected from the desi breed (Zebu cattle) reared at LPM section of IVRI, Izatnagar. Collected colostrum was converted aseptically into dry powder form by freeze drying at (-) 40º C with low pressure. Antioxidant content was analysed using free radical scavenging activity (DPPH assay) and total antioxidant capacity (FRAP assay).Qualitatively and quantitative immunoglobulin level was assessed by zinc sulphate turbidity and IgG estimation respectively. Average reduction in DPPH scavenging activity was found to be 35.04 % on freeze drying whereas average reduction of FRAP value was found to be 14.96 % on freeze drying. Immunoglobulin content of bovine colostrum was decreased by 23.86 % of ZST unit after freeze drying whereas with respect to IgG level, the average percentage reduction was 26.05 % after freeze drying of bovine colostrum. Present study concludes that freeze drying reduces antioxidants and immunoglobulin level of bovine colostrum