Scientific Publication

Performance of the HerpeSelect (Focus) and Kalon enzyme-linked immunosorbent assays for detection of antibodies against Herpes simplex virus type 2 using monoclonal antibody-blocking enzyme immunoassay (MAb-EIA) and clinico-virological reference standards in Brazil

author(s) Mayaud, P.. - Nascimento, M.C.. - Pannuti, C.S.. - Ferreira, S.. - Sabino, E.C.. - Hamilton, I.. - Parry, J.
from UK Foreign, Commonwealth & Development Office (FCDO)
permalink open
themes
regions
  • South America
  • Latin America and the Caribbean
  • Americas
  • World
citation

Journal of Clinical Microbiology (2007) 45 (7) 2309-2311

Publications and datasets provided via GARDIAN

Abstract

A total of 586 serum samples were used to evaluate the performance of type-specific herpes simplex virus type 2 (HSV-2) commercial enzyme-linked immunosorbent assays (ELISAs) by using the monoclonal antibody-blocking enzyme immunoassay (MAb-EIA) and a clinicovirological panel as reference standards. The Kalon and HerpeSelect ELISAs had similar sensitivities (93.5% and 93.8% compared with the results obtained by MAb-EIA, respectively, and 100% for both ELISAs compared with the results obtained with a clinicovirological panel). The Kalon ELISA had a higher specificity (96.5% and 96.8% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively) than the HerpeSelect ELISA (86.9% and 94% compared with the results obtained by MAb-EIA and with a clinicovirological panel, respectively). A higher cutoff significantly improved the specificity of the HerpeSelect ELISA

Open original