Scientific Publication

Viral Inhibition Assay: A CD8 T Cell Neutralization Assay for Use in Clinical Trials of HIV-1 Vaccine Candidates

author(s) Wong, J.. - Gotch, F.. - Ashraf, A.. - Karita, E.. - Anzala, O.. - Dally, L.. - Gilmour, J.. - Hayes, P.. - Walker, B.. - Anjarwalla, I.. - Bergin, P.. - Cashin-Cox, M.. - Cheeseman, H.. - Gill, D.. - Higgs, C.. - Kaltsidis, H.. - Piechocka-Trocha, A.. - Pozniak, A.. - Spentzou, A.. - Steel, A.
from UK Foreign, Commonwealth & Development Office (FCDO)
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Spentzou, A., Bergin, P., Gill, D., Cheeseman, H., Ashraf, A., Kaltsidis, H., Cashin-Cox, M., Anjarwalla, I., Steel, A., Higgs, C., Pozniak, A., Piechocka-Trocha, A., Wong, J., Anzala, O., Karita, E., Dally, L., Gotch, F, Walker, B., Gilmour, J., Hayes, P. Viral Inhibition Assay: A CD8 T Cell Neutralization Assay for Use in Clinical Trials of HIV-1 Vaccine Candidates. Journal of Infectious Diseases (2010) 201 (5) 720-729. (DOI: 10.1086/650492)

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Abstract

We have characterized an assay measuring CD8 T cell-mediated inhibition of human immunodeficiency virus (HIV) type 1 replication, demonstrating specificity and reproducibility and employing a panel of primary HIV-1 isolates. The assay uses relatively simple autologous cell culture and enzyme-linked immunosorbent assay, avoids generation of T cell clones, and can be performed with

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