Development of HPLC based method to study the bioactive component of Andrographolide paniculata in serum of Nicobari Fowl

Abstract

The bioactive component of andrographolide in Andrographis paniculata (Kalmegh) was quantified using HPLC. Aqueous extract prepared from 30 g & 50 g of Andrographis paniculata was used as water supplement in Nicobari fowls. The serum samples collected in 3 and 24 hours after supplementation were processed to comprise of Serum: phosphate buffer (pH 5.8): Methanol at the ratio of 1 ml: 0.5 ml: 1 ml and injected into HPLC. The concentration of andrographolide in serum from 30 g and 50 g group at 3 hours interval were 67.056 ppm and 151.31 from 50 g, respectively; while nil concentration at 24 hrs after feeding. The HPLC based method developed to quantify the andrographolide in serum is simple, sensitive and might be base reference for further studies on ethno veterinary medicine