Scientific Publication

An extremely rapid and simple DNA-release method for detection of M. tuberculosis from clinical specimens

author(s) Traore, H.. - Thomson, L.M.. - Yesilkaya, H.. - Doig, C.. - Steingrimsdottir, H.. - Garcia, L.. - Forbes, K.J.
from UK Foreign, Commonwealth & Development Office (FCDO)
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Journal of Microbiological Methods (2005) 63 (1) 95-98 (doi:10.1016/j.mimet.2005.03.005)

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Abstract

A single-step, 5-min lysis method was investigated as a rapid technique to extract genomic DNA from mycobacteria for PCR detection of M. tuberculosis directly from clinical specimens. Of 67 smear-positive clinical specimens, 64 (95.5%) were positive by PCR after this rapid extraction method

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