Scientific Publication

Genome characterization of a new strain of peanut chlorotic streak virus causing chlorotic vein banding disease of groundnut (Arachis hypogaea L.) in India

author(s) Satyanarayana, T - Broglio, E P - Sreenivasulu, P - Reddy, D V R - Nayudu, M V
from International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)
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  • Southern Asia
  • Asia
  • World
citation

Satyanarayana, T and Broglio, E P and Sreenivasulu, P and Reddy, D V R and Nayudu, M V (1995) Genome characterization of a new strain of peanut chlorotic streak virus causing chlorotic vein banding disease of groundnut (Arachis hypogaea L.) in India. Plant Pathology, 44 (6). pp. 966-970. ISSN 1365-3059

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Abstract

The double-stranded DNA of the chlorotic vein banding isolate of peanut chlorotic streak caulimovirus (PC1SV-CVB), isolated from purified virus, resolved into circular and linear molecules similar to those of other caulimoviruses. A physical map of viral DNA was constructed, which showed the PCLSV-CVB DNA to be circular and composed of approximately 8.2 kbp. A number of restriction sites were found to be shared with a similar caulimovirus, PCLSV. Nevertheless, several differences between physical maps of the 2 viruses suggested that PCLSV-CVB should be considered as a distinct strain of PCLSV. Bam HI-cleaved PCLSV-CVB DNA was cloned into pUC 118 and was infectious when cleaved from the cloning vector and inoculated onto Vigna unguiculata (cowpeas)

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