The isolation of a single spore isolate of Pasteuria penetrans and its pathogenicity on Meloidogyne javanica

Abstract

We have obtained a single spore isolate of Pasteuria penetrans, derived by allowing a single spore to attach to a secondstage juvenile (J2) of the root-knot nematode Meloidogyne javanica. By analysing DNA sequences at three different loci we have obtained evidence that the isolate is, indeed, genetically pure. We compared the ability of the single spore isolate and the parent population from which it was selected to attach to and parasitise both the original population of M. javanica on which it was isolated and a single egg mass line derived from it. There was no difference in the attachment of spores of the single spore isolate to juveniles compared to the parental population, although there were higher numbers of both attaching to J2 of the single egg mass line compared to its parental population. Judging from the numbers of egg masses and Pasteuria -infected females, the single spore isolate was less pathogenic to the parental population of M. javanica than was the parental spore population