Scientific Publication

Oligonucleotide fingerprinting reveals various probe-dependent levels of informativeness in chickpea (Cicer arietinum)

author(s) Weising, Kurt - Kaemmer, Dieter - Weigand, Franz - Epplen, Jörg T - Kahl, Günter
from International Center for Agricultural Research in the Dry Areas (ICARDA)
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Kurt Weising, Dieter Kaemmer, Franz Weigand, Jörg T. Epplen, Günter Kahl. (15/2/2011). Oligonucleotide fingerprinting reveals various probe-dependent levels of informativeness in chickpea (Cicer arietinum). Genome, 35 (3), pp. 436-442

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Abstract

Synthetic oligonucleotides complementary to simple repetitive DNA sequences were used to detect inter- and intra-specific polymorphisms in a leguminous crop plant (chickpea, Cicer arietinum) and its wild relatives. All the investigated repetitive motifs ((GACA)4, (GATA)4, (GTG)5, (CA)8, (TCC)5, (GGAT)4, and (AGTTT)4) were abundantly present and polymorphic in the chickpea genome. Different probes revealed different levels of variability. Whereas species-specific banding patterns were obtained with the (GTG)5 probe, other probes revealed differences between accessions, or even individuals. The somatic multilocus patterns were stable for all probes

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