Physical mapping of the 7D chromosome using a wheat/barley translocation line (5HS.7DL) produced in Martonvásári wheat background using microsatellite markers

Abstract

A wheat/barley translocation line was previously developed in Martonvásár from the Mv9kr1 × Igri hybrid and was identified as a 5HS.7D translocation using fluorescence in situ hybridization (FISH) and 5Hspecific barley SSR markers. The aim of the present study was to characterize the rearranged wheat chromosome and to identify the 7D chromosome segment eliminated by the translocated 5HS segment using 7D-specific SSR markers. The elimination of the 7DS terminal region was proved by three of the twenty-four tested markers. The breakpoint of the 5HS.7DS translocation was considered to be closer to the telomere than the breakpoint of known deletion lines, which provides a new physical landmark for future deletion mapping studies. The fine mapping of 7D makes it possible to localize agronomically useful genes to the precise chromosomal region of the eliminated 7DS segment, opening up the possibility of marker-assisted breeding and map-based cloning