Purification of secondary metabolites from 2 actinomycetes responsible for antagonistic activity against important fungal pathogens of chickpea and sorghum

Abstract

In our previous study, a total of 137 actinomycetes, isolated from 25 different herbal vermi-composts, were characterized for their antagonistic potential against Macrophomina phaseolina (causes charcoal rot in sorghum and dry root rot in chickpea) by dual-culture assay. Of them, eight most promising isolates (CAI-17, CAI-21, CAI-26, CAI-68, CAI-78, KAI-26, KAI-27 and MMA-32) were demonstrated to have the potential for PGP and control of charcoal-rot disease in sorghum. These were also identified as Streptomyces but different species in the 16S rDNA analysis. In the present study, the most promising isolate Streptomyces sp. MMA-32 was further evaluated for its antagonistic activity against M. phaseolina and partial purification of the active metabolite was done