Replication data for: Complete genome sequence of a potyvirus infecting yam beans (Pachyrhizus spp.) in Peru

Abstract

Towards the end of 2010, viral disease symptoms (mosaic with or without leaf deformation) were observed in a yam bean field trial at the CIP research station in San Ramon, Junin, Peru (wet tropics, 800 masl). The disease could be graft transmitted, and based on symptoms, an infection by the potyvirus bean common mosaic virus (BCMV) was suspected. Plants were tested by NCM-ELISA using potyvirus-specific antibodies (Agdia CAB 27200), to which all symptomatic plants reacted positively, whereas symptomless greenhouse-grown plants did not react. Small interfering RNA (siRNA) sequencing (Provider: Fasteris Life Sciences SA, Plan-les-Ouates, Switzerland) and assembly were performed on a sample (P. tuberosus accession CIP209014) showing severe mosaic and leaf deformation as described by Kreuze et al., except that the reads were assembled using Velvet’s Assembly Assembler (v1.1)