A simple, low‐cost technique for in situ measurement of leaf P concentration in field‐grown rice

Abstract

AbstractSimple tools for diagnosing crop nutritional status are in great demand by agricultural extension staff, particularly in low‐fertility environments. We developed the first practical method for in situ diagnosis of phosphorus (P) nutrition in field‐grown cereal crops by using a handheld colorimeter. The concentration of P in extracts from fresh leaves of lowland rice grown under various P availabilities was closely correlated with that of oven‐dried leaves measured with the standard molybdenum‐blue method in the laboratory, over a range of values from 0.319 to 1.420 mg P g −1 DW (r = 0.885**, n = 14). It takes only 3–5 min to estimate the P concentration of plant samples using the new technique. Although not as accurate as the laboratory method, the new method can easily detect P deficiency of rice in the field (and possibly other cereal crops) without requiring costly, off‐site equipment. Thus, extension staff and agronomists can easily adopt this technique